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Cell culture in-house training courses

Background

Why book an in-house course ?

  • In many situations its favourable to train all members of a lab at once. This way, everyone is involved in setting new standards for the lab and has the possibility to discuss concerns. When we train on your premises, you save travel expenses for your lab staff or collegues.
  • In-house trainings are tailored to suit your needs. Depending on your goal, the work background and training basis, we set up a specific course from the below array of topics.
  • During an in-house training, new routines and methods may be directly tested for suitability and practicability in your lab. For practical trainings a sufficient number of clean benches should therefore, be provided.
in-house courses

Topics of theoretical courses and practical trainings

To achieve optimal learning conditions, we always try to combine theoretical and practical training sections. Then, the newly established knowledge is directly transferred into practical routines and may further be commented and discussed within the training.

Our theoretical topics include:

  • Cell culture trouble shooting
  • Smart organization of cell culture labs
  • Media and supplements
  • Cell culture plastic ware (choice and comparison of flasks and dishes)
  • Hygiene
  • Aseptic technique
  • Antibiotics in cell culture
  • Contamination: bacteria, yeats or other funghi
  • Mycoplasma contamination and mycoplasma testing
  • cell lines and primary cells
  • cell culture routine methods (Thawing of cells, passaging with trypsin and alternatives, medium change, frezzing of cells)
  • Cell banking (easy to GMP level)
  • Assurance of consistent quality of cell lines and primary cells
  • Methods for cell couting and vitality testing
  • Automated cell counting in different counters
  • Growth curves
  • Passages, Population doublings, generation time
  • Equipment testing and maintenance in the cell culture lab
  • Documentation with the cell culture lab (easy to GMP level)
  • General SOPs for cell culture (einfach bis GMP)
  • SOPs for cell culture routine methods
  • Bioassays: Optimization and trouble shooting
  • Cell proliferation and vitality assays
  • Impedance measurements
  • Quality management and assurance in cell culture (QM, QA)

Our practical topics include:

  • Cell culture trouble shooting
  • Hygiene
  • Aseptic technique workshop
  • Recognize contaminations
  • Mycoplasma testing using PCR kits
  • Routine methods (thawing, medium change, passaging, freezing of cells)
  • Cell counting with hematocytometers and counters
  • Generation of growth curves
  • Calculation in cell culture
  • Evaluation of cell morphologies
  • Isolation of primary cells from the human umbilical chord (HUVEC)
  • Cell separation with magnetic beads
  • Cell proliferation, vitality and cytotoxicity tests

 

Target group

Everyone who wants to work with cell cultures or is working with cell cultures already. Each training is tailored in such a way that it fits the background of participants. QA aspects are scaled from general up to GMP level depending on the needs.

Dates and prizes

Please contact us for an offer tailored to your needs. All dates are set to suit your schedule. Usually a course can be performed as fast as 4-8 weeks after the initial contact. Depending on the level of your group, topics can also be added or removed to better fit the training necessities. Our contact details are found here.

Trainer

Dr. Nicole Kühl studied Biology at the Technical University of Karlsruhe and the University of Bremen. 1999 she completed her Ph.D. in cell and molecular biology studying the cell cycle and heat shock proteins. Dr. Kühl then began her post-doctorate work at the Academic Hospital Groningen in the Netherlands, where she specialized in research related to multiple sclerosis and worked with primary glial cells. In 2005, she continued her work at the Jacobs University Bremen, where she led a research group and lectured in biochemistry and cell biology. From 2008 to 2011, Dr. Kühl was the head of the PromoCell Academy and taught cell culture courses. Since 2012, she is a freelance trainer and consultant in the area of cell culture quality control and runs the cell culture web site InCelligence.de. Watch Dr. Kühl in a video from our MOOC.