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Cell Culture Comprehensive Course

Content & Learning Objective

This course will provide  basic knowledge and build upon your existing expertise at the same time, so that your daily cell culture work becomes safer and more efficient. Beginners as well as long-term users of cell culture often work with only limited knowledge about the "why´s" acquired from being trained quickly by a colleague or via "learning-by-doing". Also, cell culture has changed over the last years and many new options and tools are available. Besides , introducing standards will help  increase reproducibility and accuracy and understanding the "why" will help you trouble shoot your experiments.

During this course, you will have sufficient time to acquire or troubleshoot practical experience and in addition, we will introduce you to smart ideas, standards, documentation and cell banking.

PromoCell Academy

The theoretical seminars include:

  • Aseptic techniques
  • Contamination (including mycoplasmas)
  • Routine methods in cell culture (passaging, thawing, freezing)
  • Ingredients of culture media
  • FCS and reproducibility
  • Cell culture plastic ware
  • Differences between cell lines and primary cells
  • Cell counting and viability testing
  • Growth curves
  • Cell banking

The practical parts include:    

  • Aseptic working technique: workshop
  • Trypsinization, freezing, and thawing of cells
  • Cell counting and vitality stain
  • Medium exchange for adherent and suspension cells
  • Microscopy and tips for analysis
  • Recognition of contamination

Target Group

Technical and scientific staff members with no previous cell culture experience or little cell culture experience are the primary target. However, this course is also recommended for long-term cell culture users, as it helps to troubleshoot aseptic technique, gives an overview over new developments and offers many tricks and tips.

Duration

4 days

Dates and pricing

Please contact us for an offer tailored to your needs. All dates are set to suit your schedule. Usually a course can be performed as fast as 4-8 weeks after the initial contact. Depending on the level of your group, topics can also be added or removed to better fit the training necessities. Our contact details are found here.

Teacher

Dr. Nicole Kühl studied Biology at the Technical University of Karlsruhe and the University of Bremen. In 1999 she completed her Ph.D. in cell and molecular biology studying the cell cycle and heat shock proteins. Dr. Kühl then began her post-doctorate work at the Academic Hospital Groningen in the Netherlands, where she specialized in research related to multiple sclerosis and worked with primary glial cells. In 2005, she continued her work as a PI at  Jacobs University Bremen, where she led a research group and lectured in biochemistry and cell biology. From 2008 to 2011, Dr. Kühl was the head of the PromoCell Academy where she taught cell culture and quality management courses up to GMP level. Since 2012, she is a freelance trainer and consultant in the area of cell culture quality control and runs the cell culture web site www.InCelligence.de. Watch Dr. Kühl in a video from our MOOC.