Cell culture training courses

Background

Why book an in-house course ?

One defined standard for all
In many situations its favorable to train all members of a lab at once. This way, everyone is involved in setting new standards for the lab and has the possibility to discuss concerns. When we train on your premises, you save travel expenses for your lab staff or colleagues.
Train what you need
In-house trainings are tailored to suit your needs. Depending on your goal, the work background and training basis, we set up a specific course from the below array of topics.
Tailor to match your lab
During an in-house training, new routines and methods may be directly tested for suitability and practicability in your lab. For practical trainings a sufficient number of clean benches should therefore, be provided.

Course program cell culture

Topics of theoretical courses and practical trainings

To achieve optimal learning conditions, we always try to combine theoretical and practical training sections. Then, the newly established knowledge is directly transferred into practical routines and may further be commented and discussed within the training.

Our theoretical topics include:

Cell culture trouble shooting
Smart organization of cell culture labs
Media and supplements
Cell culture plastic ware (choice and comparison of flasks and dishes)
Hygiene
Aseptic technique
Antibiotics in cell culture
Contamination: bacteria, yeast or other fungi
Mycoplasma contamination and mycoplasma testing
Cell lines and primary cells
Cell culture routine methods (thawing of cells, passaging with trypsin, accutase and alternatives, medium change, freezing of cells)
Cell banking (basics up to GMP level)
Assurance of consistent quality of cell lines and primary cells
Methods for cell counting and vitality testing
Automated cell counting in different counters
Growth curves
Passages, population doublings, generation time
Equipment testing and maintenance in the cell culture lab
Documentation with the cell culture lab (basics up to GMP level)
General SOPs for cell culture (einfach bis GMP)
SOPs for cell culture routine methods
Bio-assays: Optimization and trouble shooting
Cell proliferation and vitality assays
Impedance measurements
Quality management and assurance in cell culture (QM, QA)

Our practical topics include:

Cell culture trouble shooting
Hygiene
Aseptic technique workshop
Recognize contamination
Mycoplasma testing using PCR kits
Routine methods (thawing, medium change, passaging, freezing of cells)
Cell counting with hematocytometers and counters
Generation of growth curves
Calculation in cell culture
Evaluation of cell morphologies
Isolation of primary cells from the human umbilical chord (HUVEC)
Cell separation with magnetic beads
Cell proliferation, vitality and cytotoxicity tests

Target group

Everyone who wants to work with cell cultures or is working with cell cultures already. Each training is tailored in such a way that it fits the background of participants. QA aspects are scaled from general up to GMP level depending on the needs.

Dates and prizes

Please contact us for an offer tailored to your needs. All dates are set to suit your schedule. Usually a course can be performed as fast as 4-8 weeks after the initial contact. Depending on the level of your group, topics can also be added or removed to better fit the training necessities. Our contact details are given here.

Trainer

Dr. Nicole Kühl studied Biology at the Technical University of Karlsruhe and the University of Bremen. 1999 she completed her Ph.D. in cell and molecular biology studying the cell cycle and heat shock proteins. Dr. Kühl then began her post-doctorate work at the Academic Hospital Groningen in the Netherlands, where she specialized in research related to multiple sclerosis and worked with primary glial cells. In 2005, she continued her work at the Jacobs University Bremen, where she led a research group and lectured in biochemistry and cell biology. From 2008 to 2011, Dr. Kühl was the head of the PromoCell Academy and taught cell culture as well as quality assurance and GMP courses. Since 2012, she is a freelance trainer and consultant in the area of cell culture quality assurance and GMP and runs the cell culture web site InCelligence.de. Watch Dr. Kühl in a video from our MOOC.

Dr. Kühl also works as a consultant for manufacturers of e.g. incubators, cell culture plastic consumables, cell counter and analyzers. Since 2016 Dr. Kühl acts as an external head of quality assurance for ANOVA IRM GmbH. There she acquired two legal permissions from the German regulatory bodies, one for a tissue preparation and one for an ATMP.